Eyepiece calibration
Compare a stage interval with the eyepiece divisions to establish length per division for the selected objective.
Single-axis glass scales for calibrating eyepiece divisions and camera pixels at the specimen plane.

A stage micrometer is a glass scale placed at the specimen plane. Its known intervals relate image pixels or eyepiece divisions to physical length for a defined microscope configuration.
Choose it to calibrate eyepiece divisions or image-analysis pixel scale, and to repeat that check after changing the objective, camera adapter or saved image mode.
Compare a stage interval with the eyepiece divisions to establish length per division for the selected objective.
Measure a known interval in the saved image to establish length per pixel at that magnification and image mode.
| Choice | Selection basis |
|---|---|
| Scale span | Choose an observable span long enough for repeatable endpoint judgement within the usable field. |
| Division spacing | Finer divisions are useful only if adjacent marks remain separable at the chosen objective and camera sampling. |
| Reported intervals | Identify nominal dimensions, measured intervals and any uncertainty or traceability required for the result. |
The stage scale is at the specimen plane. An eyepiece reticle is inside the viewing optics and requires a calibrated conversion to specimen length.
Choose spans and interpret measurement recordsA length reference for pixels or eyepiece divisions.
Specimen-plane length references for image calibration.
Inspect the defined mark intervals, accumulated spans, line quality and identification. A nominal division size is distinct from the measured interval and its uncertainty.
The supplied item is the selected patterned glass reference. The order identifies the measured intervals and accompanying measurement records.
Linear stage micrometers provide known single-axis graduations at the specimen plane for image-scale calibration.
Measure a known interval in the same objective, zoom and camera mode used for inspection. Retain the conversion and setup record.
Scale interval, total length, numbering, substrate format and calibration record.
Measure the pixel span of a known graduation distance in the same optical configuration, calculate length per pixel and retain the configuration.